Algorithm could enable visible light-based medical imaging

Oct. 3, 2016
A new technique recovers visual information from light that has scattered because of interactions with the environment.

Researchers at the Massachusetts Institute of Technology (MIT; Cambridge, MA) have developed a technique for recovering visual information from light that has scattered because of interactions with the environment, such as passing through human tissue. The technique could lead to medical imaging systems that use visible light, which carries much more information than x-rays or ultrasound waves, as well as computer vision systems that work in fog or drizzle for use in self-driving cars.

In the researchers' experiments, they fired a laser beam through a mask (a thick sheet of plastic with slits cut through it in a certain configuration, such as the letter A) and then through a 1.5 cm tissue phantom (a slab of material designed to mimic the optical properties of human tissue for purposes of calibrating imaging systems). Light scattered by the tissue phantom was then collected by a high-speed camera, which could measure the light's time of arrival. From that information, the researchers' algorithms were able to reconstruct an accurate image of the pattern cut into the mask.

"The reason our eyes are sensitive only in this narrow part of the spectrum is because this is where light and matter interact most," says Guy Satat, a graduate student at the MIT Media Lab and first author on the paper describing the work. "This is why x-ray is able to go inside the body, because there is very little interaction. That's why it can't distinguish between different types of tissue, or see bleeding, or see oxygenated or deoxygenated blood."

In experiments, the researchers fired a laser beam through a mask—a thick sheet of plastic with slits cut through it in a certain configuration, such as the letter A—and then through a 1.5 cm tissue phantom, a slab of material designed to mimic the optical properties of human tissue for purposes of calibrating imaging systems. Light scattered by the tissue phantom was then collected by a high-speed camera, which could measure the light's time of arrival. (Image credit: Camera Culture Group/MIT)

The new system relies on a pulsed laser that emits ultrashort bursts of light and a high-speed camera that can distinguish the arrival times of different groups of photons. When a light burst reaches a scattering medium, such as a tissue phantom, some photons pass through intact, some are only slightly deflected from a straight path, and some bounce around inside the medium for a comparatively long time. The first photons to arrive at the sensor have thus undergone the least scattering, while the last to arrive have undergone the most.

Where previous techniques have attempted to reconstruct images using only those first unscattered photons, the researchers' technique uses the entire optical signal—so the research team has dubbed it all-photons imaging. When light arrives at the camera from only one point in the visual field, the first photons to reach the camera pass through the scattering medium as a single illuminated pixel in the first frame of the movie. The next photons to arrive have undergone slightly more scattering, so in the second frame of the video, they show up as a small circle centered on the single pixel from the first frame. With each successive frame, the circle expands in diameter, until the final frame just shows a general, hazy light.

An illustration shows the researchers' experimental setup. The data captured by the camera can be thought of as a movie—a two-dimensional image that changes over time. (Illustration credit: Camera Culture Group/MIT)

The problem, of course, is that in practice the camera is registering light from many points in the visual field, whose expanding circles overlap. The job of the researchers' algorithm is to sort out which photons illuminating which pixels of the image originated where. The first step is to determine how the overall intensity of the image changes in time. This provides an estimate of how much scattering the light has undergone: If the intensity spikes quickly and tails off quickly, the light hasn't been scattered much. If the intensity increases slowly and tails off slowly, it has.

On the basis of that estimate, the algorithm considers each pixel of each successive frame and calculates the probability that it corresponds to any given point in the visual field. Then it goes back to the first frame of video and, using the probabilistic model it has just constructed, predicts what the next frame of video will look like. With each successive frame, it compares its prediction to the actual camera measurement and adjusts its model accordingly. Finally, using the final version of the model, it deduces the pattern of light most likely to have produced the sequence of measurements the camera made.

One limitation of the current version of the system is that the light emitter and the camera are on opposite sides of the scattering medium. That limits its applicability for medical imaging, although Satat believes that it should be possible to use fluorescent particles known as fluorophores, which can be injected into the bloodstream and are already used in medical imaging, as a light source. And fog scatters light much less than human tissue does, so reflected light from laser pulses fired into the environment could be good enough for automotive sensing.

Full details of the work appear in the journal Scientific Reports; for more information, please visit http://dx.doi.org/10.1038/srep33946.

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