Carl Zeiss’ Dual Camera module captures multichannel images simultaneously

The Dual Camera module for the AxioVision image analysis software from Carl Zeiss (Thornwood, NY) is intended for all users in the life sciences, including cell biologists, virologists and physiologists who examine fast intracellular processes with maximum resolution in both time and space. Users can simultaneously acquire images with two cameras and their highly precise synchronization within nanoseconds. The camera parameters such as exposure time or contrast can be set independently.

The Dual Camera module for the AxioVision image analysis software from Carl Zeiss permits the examination of fast intracellular processes with maximum resolution in both time and space.

Dual Camera applications comprise two identical cameras with adapter, suitable filters, and a software module. Capturing images of biological samples with two different wavelengths in two channels permits the measurement of emission ratio imaging (Indo-1), fast FRET (Förster Resonance Energy Transfer) examinations, and the imaging of cellular transport processes in cell cultures, tissues, or organisms. Simultaneously imaging tissue and cell structures using infrared transmitted-light techniques like IR-DIC (differential interference contrast) and fluorescence excitation – a major requirement for electrophysiological work in neurobiology – is a further application that benefits from the Dual Camera module.

Due to the simultaneous capture of two separate images, the Dual Camera module provides a higher speed of capture than techniques where images are only captured sequentially with one camera. Furthermore, artifacts that can occur in sequential captures of double-stained structures with only one camera are effectively prevented, as are errors in ratiometric measurements of two emission channels (e.g. with Indo-1).