Super-resolution Fourier Ptychographic Microscopy

Super-resolution Fourier Ptychographic Microscopy (FPM)

FPM addresses the fundamental trade-off between resolution and field of view in conventional microscopy by combining principles of structured illumination, ptychography, and phase retrieval. This technique enables high-resolution, wide-field imaging using low numerical aperture, and low magnification objective lenses.

Super-resolution Fourier Ptychographic Microscopy Principles

FPM utilizes an array of programmable LEDs for illumination from different angles, expanding the frequency domain bandwidth by overlapping pupil functions. Key components include:

• LED array illumination: Provides angularly varying illumination to capture multiple low-resolution images of the sample from different incident angles.
• Low-NA objective lens: Captures wide field-of-view images at low resolution, which are later computationally enhanced.
• Digital camera: Records the series of low-resolution intensity images corresponding to different illumination angles.
• Computational reconstruction algorithms combine the captured low-resolution images in the Fourier domain to synthesize a high-resolution image with both amplitude and phase information. 

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