Metabolic FLIM with Simultaneous pH Imaging

Nov. 15, 2024

The Becker & Hickl DCS-120 MP multiphoton FLIM system combines metabolic imaging by NAD(P)H with pH imaging by BCECF (2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein). The system uses a femtosecond fibre laser with an emission wavelength of 785 nm. Two-photon excitation with this laser excites both NAD(P)H and BCECF at reasonable efficiency. The fluorescence of the two compounds is split spectrally on the emission side and recorded in two parallel TCSPC FLIM channels.

Learn More

Continue Reading

Is your Quantum 2.0 solution stable enough?

Optical design software: From nano to system scale

Sponsored Recommendations

Brain Computer Interface (BCI) electrode manufacturing

Jan. 31, 2025

Learn how an industry-leading Brain Computer Interface Electrode (BCI) manufacturer used precision laser micromachining to produce high-density neural microelectrode arrays.

Electro-Optic Sensor and System Performance Verification with Motion Systems

Jan. 31, 2025

To learn how to use motion control equipment for electro-optic sensor testing, click here to read our whitepaper!

How nanopositioning helped achieve fusion ignition

Jan. 31, 2025

In December 2022, the Lawrence Livermore National Laboratory's National Ignition Facility (NIF) achieved fusion ignition. Learn how Aerotech nanopositioning contributed to this...

Nanometer Scale Industrial Automation for Optical Device Manufacturing

Jan. 31, 2025

In optical device manufacturing, choosing automation technologies at the R&D level that are also suitable for production environments is critical to bringing new devices to market...