Metabolic FLIM with Simultaneous pH Imaging

Nov. 15, 2024

The Becker & Hickl DCS-120 MP multiphoton FLIM system combines metabolic imaging by NAD(P)H with pH imaging by BCECF (2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein). The system uses a femtosecond fibre laser with an emission wavelength of 785 nm. Two-photon excitation with this laser excites both NAD(P)H and BCECF at reasonable efficiency. The fluorescence of the two compounds is split spectrally on the emission side and recorded in two parallel TCSPC FLIM channels.

Learn More

Continue Reading

Optical design software: From nano to system scale

Quantum key distribution: Secure optical communications

Sponsored Recommendations

How to Tune Servo Systems: Force Control

Oct. 23, 2024

Tuning the servo system to meet or exceed the performance specification can be a troubling task, join our webinar to learn to optimize performance.

Laser Machining: Dynamic Error Reduction via Galvo Compensation

Oct. 23, 2024

A common misconception is that high throughput implies higher speeds, but the real factor that impacts throughput is higher accelerations. Read more here!

Boost Productivity and Process Quality in High-Performance Laser Processing

Oct. 23, 2024

Read a discussion about developments in high-dynamic laser processing that improve process throughput and part quality.

Precision Automation Technologies that Minimize Laser Cut Hypotube Manufacturing Risk

Oct. 23, 2024

In this webinar, you will discover the precision automation technologies essential for manufacturing high-quality laser-cut hypotubes. Learn key processes, techniques, and best...