The MoFlo Astrios EQ six-way jet-in-air cell sorter from Beckman Coulter Life Sciences (Indianapolis, IN) features the company's enhanced dual forward scatter (eFSC) technology for simultaneous sorting and detection of particles from 200 nm to 30 µm in diameter. Intellisort II beadless drop-delay technology eliminates the need for bead-based drop-delay routines. In fully configured instruments, users can reduce compensation requirements through the use of up to seven spatially separated lasers across 32 parameters simultaneously (out of a palette of 51 parameters). Six of seven laser choices can be configured as the primary laser used in eFSC detection. Dual eFSC permits measurement of scatter characteristics across three orders of magnitude simultaneously. Choice of wavelength extends eFSC detection within 405-640 nm, allowing small- and large-particle detection. Single-cell applications for the system include circulating tumor cells, drug discovery, rare event analysis, stem and neuronal cells, and ecological and physiological microbes.
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