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    • Jim Passalugo

    Business Line Director, Semrock

    Jim Passalugo is business line director at Semrock, a unit of IDEX Health & Science LLC (Rochester, NY).

    (All panels reproduced with permission per Creative Commons open-access licensing terms [14])
    FIGURE 1. A point source of light in the focal plane gets imaged by the microscope objective as an airy disc surrounded by a concentric ring pattern resulting from diffraction (a); when projected in three dimensions, these airy patterns (top panels) comprise PSF (lower panels; b).12, 13 The width and depth of these PSFs dictate maximize x-, y-, and z-resolution, respectively, with higher NA lenses generating smaller airy patterns and PSFs, and thus greater resolution (c).
    Detectors & Imaging

    Optics/Advanced Microscopy: Practical considerations for advanced microscopy

    Prashant Prabhat, Ph.D.
    Oct. 13, 2016
    Ensuring that data generated by advanced imaging methods faithfully represent the biology being studied requires an understanding of key considerations.