Håkan Karlsson

CEO and Cofounder, Cobolt (HÜBNER Photonics)

[email protected]https://www.coboltlasers.com

Håkan Karlsson is CEO and cofounder of Cobolt, a part of HÜBNER Photonics (Solna, Sweden).

FIGURE 1. The spectral demixing principle shows normalized spectra of two typical red dyes, AF647 and CF680, with the long-pass dichroic filter at 700 nm to separate the two emission (a); an example of PSF recordings on Cam1 and Cam2 (b, top), zoom-in (b, middle), and 2D Gaussian PSF representation to evaluate center position and photon counting (b, bottom); photon ratio distribution and an example of ranges chosen for ƛ assignment (c); an optical pathway adopted for the spectral separation, with a first dichroic separate excitation from emission—the emission light is then split with a second long-pass dichroic into the two cameras (d); and an example of image reconstruction of a Cos7 cell labeled for tubulin AF647 and Clathrin CF680 (e).

STORM made easy for high-throughput research

Aug. 18, 2021

Single-molecule localization microscopy (SMLM) methods are now vital to accessing biological information at the nanoscale. Now, a novel solution involving just a single laser ...

FIGURE 1. Detector sensitivity vs. wavelength for silicon (Si) and InGaAs photodiodes.

Lasers & Sources

How to choose a laser for Raman spectroscopy

Elizabeth Illy

Aug. 1, 2018

Here, in detail, are the lasers that can be used for Raman spectroscopy and why.

An example of an image taken in a single-molecule localization microscopy (SMLM) setup from the Department of Biotechnology & Biophysics at Julius-Maximilian-University of Würzburg; the three-color image is a BPAE cell with mitotracker (red), actin (cyan), and nucleus (DAPI; blue) staining (recording time: 4 s per channel, with 2048 × 2048 pixel image detail).

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Fluorescence Microscopy/Illumination: Highly stable multi-line lasers for next-generation imaging

Elizabeth Illy

Feb. 14, 2018

Advances in laser manufacturing are enabling extremely compact multi-line illumination devices that ease instrumentation design and serve researchers' needs for fuss-free reliability...