William Telford

Senior Associate Scientist, Experimental Transplantation and Immunology Branch

[email protected]https://ccr.cancer.gov/experimental-transplantation-and-immunotherapy-branch

William Telford, Ph.D. has more than 20 years experience in flow cytometry. He is extensively involved in both national and international cytometry education programs, including the National Flow Cytometry Resource Flow Cytometry Workshops (as a sustaining faculty member) and the International Society for Advancement of Cytometry, where he has taught numerous courses, tutorials and workshops over the last 10 years. He maintains a small independent research and development program in the Facility aimed at both hardware and wetware development, particularly in the area of novel laser technology, and has published extensively in this area.

FIGURE 1. A schematic shows the operation of a basic flow cytometer. Cells are introduced into a laser beam in a liquid stream by hydrodynamic focusing, either with a nozzle or enclosed quartz flow cell. Signal collection optics collect excited fluorescence signals, which are steered to PMTs using dichroic mirrors and narrow bandpass filters. Modern instruments can utilize fiber optics for both laser delivery and signal collection.

Lasers & Sources

Lasers for the Biosciences: Novel ultraviolet 320 nm laser source enhances flow cytometry

Marco Koschorreck

Sept. 13, 2017

A 320 nm laser makes high-dimensional flow cytometry easier and more economical.