Overcoming the limitations of fluorescence microscopy with shadow imaging

This webinar was originally held on June 5th, 2024, and is now available for on demand viewing.

Duration: 1 hour

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Summary

Fluorescence microscopy remains one of the single, most widely applied experimental approaches in neuroscience. While the involved targeted labeling strategies are a key strength of fluorescence microscopy, they reciprocally impose general limitations on the possible types of experiments and analyses.

Fluorescence microscopy shadow imaging is a recent development that overcomes some of these limitations. It relies on labeling not the observed membrane-bound cellular structure, but instead its surroundings to effectively provide a negative contrast image.

The speaker will discuss the current trajectory of the fluorescence microscopy shadow imaging technique in neuroscience and highlight its ease of application and versatility.

Speaker

Jan Tonnesen
Associate Professor
Instituto Biofisika (CSIS/UPV)

My group at the Institute of Biophysics in Bilbao investigates neuronal excitability in settings of plasticity and disease, with a focus on the nano-scale physiology of neural cells. We apply advanced functional techniques in live brain tissue, including STED and 2-photon microscopy, as well as electrophysiology. In my previous postdoctoral work, I have primarily addressed the functional role of dendritic spines by means of STED microscopy, which I have been technically developing toward neurophysiology applications. I have also worked extensively with optogenetics in experimental epilepsy models, and with cell replacement therapy in experimental Parkinson's disease.

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Can shadow imaging help overcome the limitations of fluorescence microscopy?

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