Advantages of sub 50 femtosecond pulses in multiphoton microscopy

One of the main advantages of using ultrafast femtosecond fiber lasers is the high peak power that they offer, which is a result of their ultra-short pulse duration. This high peak power makes these lasers ideal for a wide range of applications, including multiphoton microscopy. In this white paper we show that sub 50 fs pulse durations provide unprecedented multiphoton conversion efficiencies. This in turn helps push frontiers in label-free multiphoton applications, especially in Third Harmonic Generation imaging since it enables extended live-cell imaging and time-lapse measurements with a high level of sensitivity.