Optimizing Illumination with Optical Filters in Fluorescence and Raman Systems

Assuring a good signal-to-noise ratio in systems for cell fluorescence or Raman signal detection depends strongly on the details of the excitation or illumination system. This Technical Note summarizes optimal approaches when using the three main types of illumination sources in common use for fluorescence – broadband, white-light sources (such as arc lamps), LED-based light engines, and lasers.

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