BH’s FLIM technique uses a combination of single-photon detection and confocal or multiphoton laser scanning. By doing so, it combines the most accurate techniques of resolving optical data in space and in time. Time-correlated single-photon detection delivers fluorescence decay data at ideal sensitivity and unprecedented time resolution, and scanning delivers three-dimensional images that are free of lateral and longitudinal scattering and out-of-focus blur. By combining these features, bh FLIM outperforms any camera-based FLIM technique in image quality and time resolution.
(Image credit: Leica Microsystems)
(Image credit: Horiba Scientific)
(Image credit: Prospective Instruments)
(Image credit: Excelitas PCO)
(Image credit: Zarbeco)
