Optogenetics technique discovers neurons that promote REM sleep

Seeking to unravel the mystery about why rapid eye movement (REM) sleep (also known as dream sleep) increases when the room temperature is just right, a team of neuroscientists at the University of Bern (Bern, Switzerland) used optogenetics to show that melanin-concentrating hormone neurons within the hypothalamus increase REM sleep when the need for body temperature defense is minimized, such as when sleeping in a warm and comfortable room temperature. These data have important implications for the function of REM sleep.

Sleep cycles between two very different states of sleep. Upon falling asleep, non-REM sleep occurs, where breathing is slow and regular and movement of limbs or eyes are minimal. Then, approximately 90 minutes later, REM sleep begins, when breathing becomes fast and irregular, limbs twitch, and eyes move rapidly. In REM sleep, the brain is highly active, but the ability to thermoregulate or maintain constant body temperature is lost.

"This loss of thermoregulation in REM sleep is one of the most peculiar aspects of sleep, particularly since we have finely tuned mechanisms that control our body temperature while awake or in non-REM sleep", says Markus Schmidt of the Department for BioMedical Research (DBMR) of the University of Bern, and the Department of Neurology, Inselspital, Bern University Hospital. On the one hand, the findings confirm a hypothesis proposed earlier by Schmidt, senior author of the study, and on the other hand represent a breakthrough for sleep medicine.

In his hypothesis, Markus Schmidt suggested that REM sleep is a behavioral strategy that shifts energy resources away from costly thermoregulatory defense toward, instead, the brain to enhance many brain functions. According to this energy allocation hypothesis of sleep, mammals have evolved mechanisms to increase REM sleep when the need for defending body temperature is minimized or, rather, to sacrifice REM sleep when cold.

"My hypothesis predicts that we should have neural mechanisms to dynamically modulate REM sleep expression as a function of our room temperature," says Schmidt. Neuroscientists at the DBMR at the University of Bern and the Department of Neurology at Inselspital, Bern University Hospital, now confirmed his hypothesis and found neurons in the hypothalamus that specifically increase REM sleep when the room temperature is just right.

The researchers discovered that a small population of neurons within the hypothalamus, called melanin-concentrating hormone (MCH) neurons, play a critical role in how we modulate REM sleep expression as a function of ambient (or room) temperature. The researchers showed that mice will dynamically increase REM sleep when the room temperature is warmed to the high end of their comfort zone, similar to what has been shown for human sleep. However, genetically engineered mice lacking the receptor for MCH are no longer able to increase REM sleep during warming, as if they are blind to the warming temperature. The authors used optogenetics techniques to specifically turn on or off MCH neurons using a laser light time locked to the temperature warming periods. Their work confirms the necessity of the MCH system to increase REM sleep when the need for body temperature control is minimized.

"Our discovery of these neurons has major implications for the control of REM sleep," says Schmidt. "It shows that the amount and timing of REM sleep are finely tuned with our immediate environment when we do not need to thermoregulate. It also confirms how dream sleep and the loss of thermoregulation are tightly integrated."

REM sleep is known to play an important role in many brain functions, such as memory consolidation. REM sleep comprises approximately one quarter of our total sleep time. "These new data suggest that the function of REM sleep is to activate important brain functions specifically at times when we do not need to expend energy on thermoregulation, thus optimizing use of energy resources," says Schmidt.

Full details of the work appear in the journal Current Biology.