Iain Johnson

Director of Technical Support, Lumencor

Iain Johnson is Director of Technical Support at Lumencor (Beaverton, OR).

Coupling of a solid-state light engine to an inverted fluorescence microscope. Locations 1–5 marked in yellow correspond to the points in the light path used for throughput measurements reported in the table. The green line shows the direction of the light path from the collimating adapter input to the sample plane.

Optimizing solid-state illumination, from source to sample plane

Dec. 10, 2020

When photons from a light engine are getting lost on their way to the microscope’s sample plane, follow this path to find the failure point(s).

FIGURE 1. A four-source light engine allows electronic selection and combination of the discrete spectral outputs of solid-state sources (1-4) according to application requirements, where the spectral output of each source can be refined by filtering and, optionally, a reference photodiode can monitor light engine output to provide feedback control.

Lasers & Sources

Bioanalytics/Illumination: Just-right light - Inherently adaptive for application-specific needs

Iain Johnson

Feb. 23, 2017

Generic benefits of solid-state light engines are key reasons these next-gen sources are increasingly replacing incandescent-lamp sources in bio applications.