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    • Prashant Prabhat, Ph.D.

    Content Dam Bow Bios O T Prashantprabhat
    Business Line Leader for Semrock Catalog

    Prashant Prabhat, Ph.D. is Business Line Leader of Catalog Business at Semrock. Since 2008 he has been an Applications Scientist at Semrock. He earned his Ph.D. in Electrical Engineering from the University of Texas at Dallas and completed graduate research at Ward Lab at the University of Texas Southwestern Medical Center. It was there that he invented multifocal plane microscopy (MUM), a technique that allows for real-time visualization of fast cellular dynamics in 3D. MUM has enabled the discovery of novel trafficking pathways in cells, and formed the basis for 3D super-resolution techniques. At Semrock, he advises researchers on optical filter issues from a systems perspective, and helps develop the company’s product roadmap. As a new venture manager, he also has led the development of a computer controlled tunable filter system.

    FIGURE 1. Multicolor switched excitation and emission light paths in a fluorescence microscope differ according to configuration: Pinkel uses fixed multiband dichroic and multiband emission filters (a), Sedat uses a fixed multiband dichroic filter and a high-speed emission filter wheel (b), and Incite uses a high-speed simultaneous dichroic and emission filter switcher (c).
    Detectors & Imaging

    Fluorescence Imaging/Microscopy: Improving fluorescence signal in time-sensitive experiments

    Prashant Prabhat, Ph.D.
    April 1, 2018
    High-speed filter switching provides improvements equal to doubling or quadrupling of camera quantum efficiency, with reduced risk of specimen phototoxicity.
    (All panels reproduced with permission per Creative Commons open-access licensing terms [14])
    FIGURE 1. A point source of light in the focal plane gets imaged by the microscope objective as an airy disc surrounded by a concentric ring pattern resulting from diffraction (a); when projected in three dimensions, these airy patterns (top panels) comprise PSF (lower panels; b).12, 13 The width and depth of these PSFs dictate maximize x-, y-, and z-resolution, respectively, with higher NA lenses generating smaller airy patterns and PSFs, and thus greater resolution (c).
    Detectors & Imaging

    Optics/Advanced Microscopy: Practical considerations for advanced microscopy

    Prashant Prabhat, Ph.D.
    Oct. 13, 2016
    Ensuring that data generated by advanced imaging methods faithfully represent the biology being studied requires an understanding of key considerations.
    (Image courtesy of Mary Raven, University of California, Santa Barbara)
    A cellular sample imaged using an Olympus microscope equipped with a Spectra-X LED light engine from Lumencor and Semrock's LED-DA/FI/TR/Cy5-4X-A filter set.
    Detectors & Imaging

    Fluorescence Microscopy: Optimizing the imaging performance of LED light engines

    Prashant Prabhat, Ph.D.
    Aug. 6, 2015
    LED light engines are becoming increasingly popular for fluorescence microscopy, but standard filter sets—including those packaged with the sources—typically limit their performance...